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Addition of lactococcin 972 to exponentially growing sensitive cultures of Lactococcus lactis resulted in cell elongation and widening. Thin sections revealed that septum invagination was blocked. Cell growth progressed until most cells showed equatorial constriction and even initial deposition of material at the septum ring, although cell division did not proceed any further. The increase in the incorporation of labelled precursors into the cell wall shifted from an exponential to a linear mode in treated cultures, subsequently being arrested. Gross degeneration of the cells was observed prior to cell death, followed by slow lysis of the culture. In contrast, stationary-phase cultures remained unaffected.

Lactococcin 972, a bacteriocin that inhibits septum formation in lactococci