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Using enrichment methods, a new bacteriophage (#MI) was isolated, which is capable of generalized transduction in Ennrinia carotovora subsp. atroseptica (Eca) strain SCR11043. #MI is probably a virulent phage and contains double- stranded DNA of approximately 43 kb. Transduction frequencies for a number of chromosomal markers and plasmid pHCP2 were established, and conditions for transduction optimized. UV irradiation of the lysates prior to transduction enhanced the transduction frequency. #MI infected over 25% of Eca strains tested and so may be useful both for the genetic analysis of a number of Eca isolates and for the transductional transfer of selectable markers between strains.

Generalized transduction in the potato blackleg pathogen Erwinia carotovora subsp. atroseptica by bacteriophage M1