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SUMMARY: Certain anaerobic bacteria from human faeces were found to be able to hydrogenate cholesterol in vitro; some also decreased to a marked degree the total amount of sterols in the incubation mixture. Other organisms did not hydrogenate or decrease the amount of sterols under the conditions chosen; among these were; Clostridium welchii, C. sporogenes, Bacterium bifidum, various streptococci and micrococci, Escherichia coli, Aerobacter aerogenes. A ‘germ-free’ filtrate from human faeces was inactive. In a series of incubated samples, where the Tschugaeff reaction was applied to the non-saponifiable matter, an orange colour developed, suggestive of the presence of lathosterol.

Conversion of Cholesterol into Coprosterol by Bacteria in vitro