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Summary: Three previously reported Tn5 mutants of Methylobacterium extorquens AM1, Cou-1, Cou-3 and Cou-6, which are able to grow on methylamine but not methanol, were characterized by biochemical analyses and complementation tests using two genomic libraries of M. extorquens AM1. We have designated the genes defective in these mutants as cou-1, cou-3 and cou-6 and mapped the site of Tn5 insertion in each. Biochemical results showed that two of these methanol oxidation (Mox) mutants, Cou-1 and Cou-3, are phenotypically similar to the previously identified MoxE class of mutant while Cou-6 resembled the MoxD class. Complementation tests and mapping the site of the Tn5 insertions indicated that cou-1 is another Mox gene linked to moxE and that cou-6 is linked to moxD. The Tn5 insertion in cou-3 mapped within the moxJ gene and therefore is the first detected mutation of the moxJ gene which was identified from expression studies. A MoxE mutant of M. extorquens AM1 was complemented by two different cosmid clones; one carried the moxE gene and the other contained two mox gene clusters, moxFJGI and moxAKLB. Hybridization experiments indicated that the moxE gene was not present on this latter clone, and it must therefore encode a gene capable of suppressing a MoxE mutation.

journal of general microbiology/journal of general microbiology

Characterization of transposon insertion mutants of Methylobacterium extorquens AM1 (Methylobacterium strain AM1) which are defective in methanol oxidation