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Surface washings were prepared from cultures of fungi grown on agar slants and the wash components were fractionated by gel-filtration HPLC. A range of low-Mr glycoproteins (less than 10,000) were detected in all the washes, and in some species several high-Mr glycoprotein components (greater than 150,000) were also present. The HPLC analyses indicated species-specific differences in the profiles. Regions of specific antigenicity within profiles were detected by monoclonal antibodies.

Detection of Low-Mr Glycoproteins in Surface Washes of Some Fungal Cultures by Gel-filtration HPLC and by Monoclonal Antibodies