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of the same year; this coincided with the drought season when only a few acacia trees were in bloom. The following observations were recorded. An increasing number of creeping worker bees was observed on the ground of the apiary. An unpleasant odour was noticed associated with two colonies : examination revealed dead larvae inside capped cells that were yellowish to light brown in colour. They were soft in consistency and mucoid when punctured. Most of the affected larvae were in the prepupal stage. The cappings of the cells containing dead broods were punctured and sunken in appearance. Dead and living brood were observed in the same comb. Only two combs had dead brood in these two infected colonies. The apiary was closely observed for six months with continuous examination of dead larvae for pathogenic bacteria. Investigations into the disease outbreak. Cappings of cells containing dead brood were removed. Both dead and apparently healthy larvae were aseptically removed, placed in sterile containers and immediately transferred to the laboratory, where they were surface sterilized by dipping them in 70% (v/v) ethanol, allowed to dry and crushed between sterile slides. Larval contents were streaked on several freshly prepared 10% (v/v) sheep blood agar (2.5%, w/v, nutrient agar; Difco) plates which were incubated at 35 "C aerobically and examined at 24 h intervals for 7 d before being discarded as negatives. Direct smears prepared from dead larvae and from the growth obtained on blood agar were fixed and stained with Gram's stain. Isolated organisms were subjected to biochemical tests according to Davis & Ewing (1964), Ewing et al. (1969) and Cowan (1957). They were biotyped according to Grimont et al. (1977) and Grimont & Grimont (1978). Other confirmatory tests, including DNAase

Properties of Serratia marcescens Isolated from Diseased Honeybee (Apis mellifera) Larvae