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Gas chromatography was used to determine the long-chain fatty acid composition of 33 strains representing 17 genera of the Saccharomycetaceae, Endomycetaceae, Metschnikowiaceae and Saccharomycodaceae. The similarities between the strains were calculated on the basis of fatty acid composition, resulting in three groups. Group I strains contained oleic acid (C18:1) as the major fatty acid, while group II strains had substantial proportions of both oleic acid and linoleic acid (C18:2). Strains of group III were characterized by a high percentage of palmitoleic acid (C16:1). The one strain of Metschnikowia reukaufii studied fell within the range of variation of group II, but it contained a slightly higher proportion of palmitoleic acid than the other strains in this group.

Fatty Acid Composition as a Guide to the Classification of Selected Genera of Yeasts Belonging to the Endomycetales