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Azospirillum lipoferum strain D-2 produces the phenolate siderophores 2,3-dihydroxybenzoic acid, 3,5-dihydroxybenzoic acid and salicylic acid under iron-starved conditions. Lysine and leucine were identified as the amino acid conjugates of the siderophores. The concentration of siderophores in the culture supernatant was maximal after 20 h growth. Iron-binding proteins were present in membranes of cultures grown under iron starvation. Iron uptake was enhanced in the presence of each of the siderophores.

Isolation and Characterization of Siderophores from Azospirillum lipoferum D-2