English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Summary: The methylotrophic yeast Hansenula polymorpha was grown in a chemostat with a medium containing a mixture of glucose (C6) and methanol (C1) (87·8% C6: 12·2% C1, w/w) as sole carbon source and NH4+ as sole nitrogen source. At a constant growth rate (D 0·10 h-1) the influence of the carbon: nitrogen ratio (C:N) of the inflowing medium on the cellular and enzymic composition of the cells was studied. Three distinct growth regimes were recognized. A medium with a C:N ratio 31 resulted in nitrogen-limited growth (low protein but high carbohydrate content of the cells) and the cells metabolized only glucose. A transition growth regime was observed during growth on media with intermediate C:N ratios (12 31). When assessed from both substrate consumption and cellular composition, growth was double-substrate (carbon and nitrogen)-limited. In this transition growth regime, changes in carbon metabolism and the cellular and enzymic composition of the cells were found. With increasing C:N ratios in the growth medium a gradual repression of the synthesis of methanol-assimilating and dissimilating enzymes was found. This effect was most pronounced for alcohol oxidase, and as a consequence the cells switched from the utilization of the carbon substrate mixture to growth on glucose alone. The data presented suggest that the range within which double-substrate-limited growth can be expected is predictable from the composition of cells grown under single substrate limitation.

microbiology

Influence of the Carbon: Nitrogen Ratio of the Growth Medium on the Cellular Composition and the Ability of the Methylotrophic Yeast Hansenula polymorpha to Utilize Mixed Carbon Sources