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SUMMARY: Mutants of Pseudomonas facilis impaired in lithoautotrophic metabolism (Aut-) were obtained by Tn5 mutagenesis, mitomycin C treatment or incubation at sublethal temperature. A colony assay provided a fast screening method for hydrogenase-negative mutants. Three classes of mutants were identified: the first had lost the ability to grow autotrophically with H2 and heterotrophically with NO3- as nitrogen source; the second lacked both hydrogenase and ribulose-1,5-bisphosphate carboxylase activity but nitrate metabolism was unaffected; the third retained the activities of the key enzymes of lithoautotrophic metabolism but failed to grow with H2 and CO2. All of the mutants showed the plasmid pattern of the wild-type.

Mutants of Pseudomonas facilis Defective in Lithoautotrophy