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The isolation of Nectria galligena mutants resistant to benzoic acid (BA), the phytoalexin of immature apples, is reported. Those in which pathogenicity on mature apples was similar to the wild-type were inoculated in immature fruits. One mutant resistant to BA was more aggressive than the wild-type in young apples at the end of their maturation cycle. Aggressiveness of this mutant and the wild-type could be increased by growing the fungus before inoculation into the fruit on a medium supplemented with BA. Amounts of BA in mutant-infected and wild-type- infected tissues were similar. We conclude that BA is involved in the resistance of immature apples infected by N. galligena.

Induction of Nectria galligena Mutants Resistant to Benzoic Acid and Study of Their Aggressiveness Towards Immature Apples