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The design, fabrication and use of a flow cell that allows rapid displacement of media viewed by short working distance, high power objectives are described. The cell has a small internal depth (about 0.04 cm), small volume (about 0.05 ml), and is chemically inert. It has been tested extensively in studies of tethered bacteria.

A Miniature Flow Cell Designed for Rapid Exchange of Media Under High-power Microscope Objectives