English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

SUMMARY: Saccharomyces rouxii cells were maintained on yeast extract/neopeptone/glucose medium (YNG). Experimental medium was depleted of endogenous metal ions by repeated passage of YNG over a chelating ion-exchange resin. This treatment did not compromise the glucose or vitamin content but provided a basal medium to which essential salts could be added in defined concentrations. Accordingly it was possible to demonstrate growth requirements by this yeast species for K+, Mg2+, Zn2+ and Fe3+. Na+ would not replace K+. Additions of 1.0 μ;M-Ca2+, NH4+ and Na+, or 0.1 μ;M-Ni2+, Sn2+, Al3+, Co2+ or Cr3+ to a minimal, reconstituted medium were without effect. Cu2+ and Mn2+ additions were not required for growth but, like Mg2+, Zn2+ and Fe3+, they positively affected the biosynthesis of periplasmic acid phosphatase. Cells from iron-depleted medium had only 1 unit (g dry wt)-1 of native acid phosphatase activity although this could be stimulated 10-fold by addition of FeCl3 to washed cell suspensions before enzyme assay. Additions of 10-60 μ;M-FeCl3 to untreated YNG medium progressively increased the concentration of acid phosphatase after 3 d of culture to an upper value of 75 units (g dry wt)-1, and also increased the amount secreted into the growth medium. While unfortified YNG supported cells with only 28% of their acid phosphatase expressed (the bulk being subject to activation by Fe3+ in the assay medium) the cells from YNG fortified with 60 μ;M-FeCl3 displayed fully activated acid phosphatase. Cells from Mg2+-depleted medium exhibited bizarre, elongated cell shapes and those from Zn2+-deficient medium appeared somewhat angular by light microscopy. Fe3+-deficient cells had apparently normal morphology.

Effects of Metal-depleted Media on the Growth and Morphology of Saccharomyces rouxii and on the Status of Periplasmic Acid Phosphatase