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Summary: Five coumarin-sensitivity mutations were assigned to loci on three linkage groups: couB352 to group I: couC356, couE353 and couF354 to group II; and couD355 to group III. Coumarin inhibited cell division, affected cell shape and induced EDTA-sensitive cell–cell adhesion in vegetative cells at lower concentrations in coumarin-sensitive mutants (1.0 to 2.5 mm) than in wild-type strains (3.0 mm). One mutant, HU609 (couB352), lysed in the presence of coumarin (1.6mm). Coumarin, thiabendazole, and cambendazole induced multiple tips in pseudoplasmodia. Coumarin, unlike thiabendazole and cambendazole, did not induce metaphase arrest or haploidization of diploids. The couA351 and couF354 coumarin-sensitivity mutations are linked to growth temperature-sensitivity mutations, tsgK21 and tsgT360 respectively, which may be pleiotropic effects of the coumarin-sensitivity mutations. Exposure of vegetative cells of strains carrying either the couA351 or the couF354 mutation to the restrictive temperature (28°C) induced cell rounding and cell–cell adhesion similar to that seen on treatment with coumarin. Wild-type strains, other coumarin-sensitive strains and other temperature-sensitive strains did not exhibit this phenotype at 28°C. Furthermore, temperature-sensitivity and coumarin-sensitivity co-reverted in temperature-resistant derivatives selected from strains carrying the couA351/tsgK21 mutation.

Genetic Analysis and Phenotypic Characterization of Effects on the Cytoskeleton of Coumarin-sensitivity Mutations in Dictyostelium discoideum