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The extracellular killer toxin of Saccharomyces cerevisiae strain 28 was concentrated by ultrafiltration of culture supernatants and purified by ion-exchange chromatography. Polyacrylamide gradient gel electrophoresis in SDS indicated that the toxin is a glycoprotein with a molecular weight of about 16000. Amino acid analysis revealed that the killer toxin contains 11 1 amino acid residues, equivalent to a molecular weight of 14045; the ratio of protein to carbohydrate in the molecule is therefore about 9 to 1. The isoelectric point of the killer toxin was pH 4.4 to 4.5. The toxin was unaffected by heating at 40 "C for 1 h and its maximum activity against sensitive yeast cells was observed at pH 5-0. Cell-free extracts prepared from well- washed cells of s. cerevisiae strain 28 were toxic for sensitive yeasts. The toxin present in these extracts (intracellular toxin) was partially purified by ultrafiltration and ion-exchange chromatography. The isoelectric points of the extracellular and intracellular killer toxin were similar.

Purification and Characterization of Extracellular and Intracellular Killer Toxin of Saccharomyces cerevisiae Strain 28