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SUMMARY: A total of 15 bacterial species were screened for the presence of the Escherichia coli K12 insertion sequences IS1, IS2 and IS3 by Southern blotting and DNA hybridization. The only positive signals obtained were for IS1 in Serratia marcescens, where the sequence was present on a plasmid, and a weak hybridization to IS3 in Erwinia carotovora. This shows that the capacity for transposition alone is not sufficient to allow the spread of sequences through a wide range of bacterial species.

Distribution of the Escherichia coli K12 Insertion Sequences IS1, IS2 and IS3 Among Other Bacterial Species