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The buoyant density of isolated mitochondria and glyoxysomes obtained from Neurospora crassa grown under different conditions was investigated by density-gradient centrifugation on Percoll. Mitochondria isolated from N. crassa in the exponential phase of growth with sucrose as the carbon source were more dense (1·077; g ml-1) than those isolated from hyphae in the stationary phase (1·069; to 1·073; g ml-1). A second population of mitochondria (density 1·059; to 1·061; g ml-1) was also present in the stationary phase. When N. crassa was grown with acetate as the carbon source, mitochondria isolated from hyphae in the exponential phase of growth were found to be less dense (1·079; g ml-1) than those present in the stationary phase (1·089; g ml-1). The glyoxysomes were found to be less dense than the mitochondria and their density decreased from 1·076; to 1·055; g ml-1 as the hyphae aged.

Changes in Density of Mitochondria and Glyoxysomes from Neurospora crassa: a Re-evaluation Utilizing Silica Sol Gradient Centrifugation