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SUMMARY: Six mutations in the spoIIA locus have been mapped by transformation crosses. The Recombination Index between mutations at opposite ends of the locus was about 0·17; all six mutations are likely to be allelic. A mutation leading to oligosporogeny was found to be closely linked on both sides to mutations leading to asporogeny. For two pairs of mutants, crosses between the members of each pair yielded no wild-type recombinants, but the mutations were found not be identical. Another of the mutations was found to be partly suppressed by the general nonsense suppressor su+3, and is therefore presumably a chain-terminating mutation.

Mapping of Six Mutations in the spoIIA Locus of Bacillus subtilis and Studies of Their Response to a Nonsense Suppressor