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The magnitudes of four signals detectable in intact cells by electron paramagnetic resonance spectroscopy, and assigned to membrane-associated protein, increase continuously during the cell cycle of Escherichia coli K12. Studies on membrane multilayers prepared from cells separated according to their age in the cycle suggest that the orientation within the membrane of the ferredoxin-type signal is invariant throughout the cycle.

microbiology

Respiratory Biogenesis during the Cell Cycle of Aerobically Grown Escherichia coli K12. The Accumulation of Iron-Sulphur Clusters and their Orientation in the Membrane