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A theoretical analysis has been made of five different media which have been used for growth of the methylotrophic yeasts Hansenula polymorpha and Candida boidinii. The media compositions were found to differ significantly in their content of trace elements. Chemostat studies revealed that low growth yields, particularly at high dilution rates, were probably due to unexpected limitations in trace elements.

Theoretical Analysis of Media Used in the Growth of Yeasts on Methanol