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We have previously described a large family of mutants of Klebsiella aerogene which were selected by continuous on xylitol and which superproduce ribitol dehydrogenase. One of these strains was found to harbour a high copy number 2.1 x 10(6) dalton plasmid. This plasmid is a deletion derivative of a low copy number 3.5 x 10(6) dalton plasmid present in the ancestral strain of K. aerogenes. However, since these plasmids do not contain the genes required for pentitol catabolism and some enzyme-superproducing strains have lost all DNA homologous to the plasmids, they are not implicated in the fast growth on xylitol. The plasmids contain regions of homology with the Escherichia coli plasmid ColE1.

Minicircular ColE1-related DNA in Strains of Klebsiella aerogenes Selected for Fast Growth on Xylitol