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A selection technique based on low-speed centrifugation of an exponentially growing culture and a starvation induction method for obtaining synchronous cultures of Acanthamoeba castellanii were compared. The selection method gave the highest levels of division and synchrony. Amoebae which were induced to encyst at different times during the growth cycle by the addition of MgCl2 to the cultures differed in their ability to initiate encystation, indicating that there may be a relationship between the position of a cell in the growth cycle and its competence to initiate differentiation. The patterns of protein and ribose synthesis which were observed during synchronous growth indicate that the changes which occur in the levels of these materials during encystation may not be unique to the differentiation process.

Synchronous Cultures of Acanthamoeba castellanii and Their Use in the Study of Encystation