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A spectrophotometric technique is described by which uptake of 2-chloromercuri-4-nitro- phenol (CMNP), an organomercurial compound toxic to fungi, may be continuously monitored for 30 min periods. The method is demonstrated using mercury-resistant and mercury-susceptible isolates of Penicillium expansum and Cladosporium cladosporioides. With P. expansum no consistent differences in uptake were recorded. Mercury-resistant isolates of C. cZadosporioides consistently absorbed less CMNP into their hyphae than did mercury-susceptible isolates.

A Spectrophotometric Technique for Recording Uptake of an Organomercurial by Mycelial Fungi