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The activities of nitrogenase, glutamine synthetase and other ammonia-assimilating enzymes were studied in bacteroids of Rhizobium leguminosarum isolated 9 to 21 d after infection. Total bacteroid numbers (per plant) increased proportionally with nitrogenase, but glutamine synthetase activity decreased with increasing age of the root nodule. Glutamine synthetase was adenylylated throughout the period of increasing nitrogenase activity and during the period of constant nitrogenase activity.

The Relationship between Nitrogenase and Glutamine Synthetase in Bacteroids of Rhizobium leguminosarum of Various Ages