English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

SUMMARY: An efficient technique for the enrichment of mutants of Mucor racemosus by differential freeze-killing is described. Ungerminated spores were resistant to freeze-killing. During germination susceptibility increased up to a maximum coinciding with the appearance of germ tubes. Under optimum conditions a thousandfold preferential survival of growth-limited spores over actively germinating spores was obtained. Using this differential killing, cultures treated with mutagen were enriched with respect to auxotrophic and temperature-sensitive mutants, with the desired mutants constituting a large fraction of the survivors of the freeze-thaw treatments. This technique may be applicable to mutant enrichment in other filamentous fungi.

Enrichment of Mutants of Mucor racemosus by Differential Freeze-killing