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Tsukamura (1 971) proposed the genus Gordona for slightly acid-fast organisms isolated from sputa of patients and soil. Since the genus was found to contain the type strain of Rhodococcus rhodochrous (ATCC 13808), the gordonae were reclassified in the genus Rhodococcus (Tsukamura, 1974), a proposal supported by Goodfellow & Alderson (1977). Previously, Tsukamura & Mizuno (1975) reported that the thin-layer chromatography of ethanol /diethy1 ether extracts, after uptake of [35S]methionine, was useful for the differentiation of mycobacterial species. We have now found that this technique is also useful for the differentiation of Rhodococcus and Nocardia.

Differentiation of Rhodococcus (Gordona) and Nocardia by Thinlayer Chromatography after Uptake of [35S]Methionine