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The spiroplasmas constitute a newly discovered group of wall-less prokaryotes, resembling mycoplasmas in morphology of colonies, ultrastructure, filtrability, requirement for cholesterol, absolute resistance to penicillin and low guanine and cytosine content of cellular deoxyribonucleic acid. Actively growing spiroplasma cultures differ from those of classical mycoplasmas in being composed mostly of long helical filaments which have a screw-like rotational and flexional movement (Cole et al., 1973). Our previous investlgation (Razin et al., 1973) showed that the cell membrane of S. citri can be readily isolated by osmotic lysis of the organisms. In gross chemical composition and ultrastructure it resembled the cell membrane of the classical mycoplasmas. The purpose of the present investigation was to provide more information on the enzymic activities and carbohydrate moieties of the S. citri membrane.

Carbohydrate Content and Enzymic Activities in the Membrane of Spiroplasma citri