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The main difficulties encountered in mycoserology are (i) the large number of rabbits required for the serological classification of a single fungal genus and (ii) the identification and quantification of antigens and antibodies in the highly complex fungal system. Anacker & Munoz (1961) found the peritoneal fluid of mice immunized with egg albumin in Freund's adjuvant could contain 2 mg antibody nitrogen ml-l, an antibody concentration which is difficult to obtain in rabbit antisera. Immune mouse peritoneal fluid has been used as a source of antibodies for plant, animal and human viruses (e.g. Herrman & Engle, 1958; Reddecliff & Ludwig, 1966; Scott, Kim & Dale, 1969; Feild & Kalter, 1972; Kiriyama & Ohsumi, 1973) and for certain bacteria (Thoen & Karlson, 1970; Bocharova & Andreeva, 1971) but not in fungus serology. Axelsen (1971, 1973) demonstrated and quantified the antigens in Candida albicans by using immune rabbit sera and the crossed immunoelectrophoresis technique. There are no reports on the use of immune mouse ascitic fluid in quantitative immunoelectrophoretic procedures. Immune ascitic fluid could be useful in fungal studies but only if the antigen-antibody reactions can be examined by quantitative immunoelectrophoresis. The purpose of our work was to investigate this possibility.

Demonstration of Antifungal Antibodies in Hyperimmune Mouse Ascitic Fluid by Means of Crossed Immunoelectrophoresis