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SUMMARY Virus infection causes an increase in the quantity of ribosomes extracted from the hypocotyls of Mung beans. Though this increase is not confined to a particular size of ribosome, presumptive virus messenger RNA is associated predominantly with polyribosomes composed of nine or more monoribosomes. METHODS Virus. A virus from cowpea (CMV; Lister & Thresh, I955) which has been shown to be related serologically to tobacco mosaic virus (TMV) was propagated in the leaves of French beans var. Prince. The virus was purified by differential centrifugation, stored as a 2 ~ solu- tion and infective RNA prepared from it by treatment with phenol (Schuster, Schramm & Zillig, I956). Infectivity assay. The relative infectivity of preparations of virus or virus RNA was determined by inoculating the leaves of tobacco plants (Nicotiana tabacum L. cv. Xanthi) hypersensitive to this virus. The plants were trimmed to three or four leaves by removing the youngest and oldest leaves and each preparation was inoculated the same number of times to all plants and all leaf positions. Mung beans. Seeds of Phaseolus aureus Roxb. were germinated in the dark in plastic boxes on a filter paper and cotton wool pad wetted with Hoagland's solution and trace elements (boric acid o'57o mg./1., manganese chloride 0"314 rag./1., cupric chloride o.21 mg./1., sodium molybdate 0"204 rag./1., zinc chloride 0.625 rag./1., EDTA ferric monosodium complex 0.720 mg./1.). Fresh humid air was pumped continually through the boxes. After the beans had been germinated for 9o to IOO hr and were approximately 6 cm. long the hypo- cotyls were inoculated manually with infective RNA in o.o6 M-phosphate buffer at pH 7"o or with buffer alone. (32P)orthophosphate incorporation. Segments 6 cm. long were cut from the hypocotyls of Mung beans at various times after inoculation (see text) and 5 g. fresh weight was placed in a

Changes in the Ribosomes Extracted from Mung Beans Infected with a Strain of Tobacco Mosaic Virus