English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

A combination of 9,10-dimethyl-1,2-benzanthracene (DMBA) application and excisional wounding on the lingual tips of Syrian Golden hamsters (Mesocricetus auratus) induces dysplastic and malignant mucosal lesions. Papillomavirus genus-specific antigen and viral particles, measuring 55 nm in diameter, were demonstrated in the nuclei of squamous cells of dysplastic lesions showing koilocytotic change. In this study, we cloned a circular genome at a single Kpnl site from one of these dysplastic lesions. The genomic sequence of this clone, consisting of 7647 bp, was shown to be that of a novel papillomavirus with a conserved genomic organization. We named the new virus hamster oral papillomavirus (HOPV). All dysplastic lesions induced by this combination of DMBA application and excisional wounding contained viral DNA. Although Southern blot hybridization analysis could not detect the HOPV genome, PCR analysis demonstrated the latent HOPV genome in the tongue and skin of an untreated hamster. These results suggest that latently present HOPV genome is reactivated by the DMBA/wounding procedures. Lingual HOPV infection may be an important model for gaining insight into the interactions between papillomavirus infection, chemical carcinogens and physical irritations in carcinogenesis or malignant transformation.

journal of general virology

Presence of a novel hamster oral papillomavirus in dysplastic lesions of hamster lingual mucosa induced by application of dimethylbenzanthracene and excisional wounding: molecular cloning and complete nucleotide sequence.