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SummaryPolyadenylated RNA was isolated from Nicotiana benthamiana plants infected with tomato golden mosaic virus (TGMV). Northern hybridization with a strand-specific probe of cloned TGMV DNA A revealed a 0.9 kb transcript with the same orientation as virion DNA. The positions of the 5′ and 3′ termini of the transcript, which were mapped by nuclease protection and primer extension techniques, indicated that it corresponded to the virus coat protein mRNA and enabled the likely functional promoter and polyadenylation signals to be identified.

Transcriptional Mapping of the Coat Protein Gene of Tomato Golden Mosaic Virus