English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

SummaryCucumber mosaic virus (CMV) strain Y from Japan was propagated in Xanthi tobacco. Virus isolated from four successive passages was analysed for its constituent RNAs by polyacrylamide gel electrophoresis (PAGE) under semi-denaturing and fully denaturing conditions. This revealed the initial presence of the 368-nucleotide satellite RNA Y-CARNA 5 (for Y-CMV-associated RNA 5) and the subsequent emergence of a small RNA in the 335-nucleotide size range, which we termed Yn-CARNA 5. The appearance of the smaller Yn-CARNA 5 correlated positively with the increasing tomato necrosis-inducing properties of tissue extracts from the later tobacco passages. After sucrose gradient ultracentrifugation of total CMV-Y RNA, the fraction containing Y-CARNA 5 and Yn-CARNA 5 was subjected to PAGE under semi-denaturing conditions, which provided highly purified gel-eluted preparations of the two CARNA 5 species. When tested for their tomato necrosis-inducing capability in the presence of helper virus genomic RNAs, Y-CARNA 5 was shown to be incapable of eliciting lethal necrosis in Rutgers tomato whereas Yn-CARNA 5 was highly necrogenic.

The 368-Nucleotide Satellite of Cucumber Mosaic Virus Strain Y from Japan Does Not Cause Lethal Necrosis in Tomato