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SUMMARY Virus-specific double-stranded replicative form (RF) RNA was isolated from cowpeas infected with cowpea mosaic virus. The RF was assayed for infectivity in a local lesion host and a systemic host. In neither host was undenatured RF infectious, although infectivity was restored upon denaturation. Cowpea mosaic virus (CPMV) is a virus of legumes whose genome consists of two separately encapsidated, positive-sense RNA molecules, both of which are required for infection (van

Double-stranded, Replicative Form RNA Molecules of Cowpea Mosaic Virus Are Not Infectious