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Intracellular polyadenylated viral RNA from cells infected by five different isolates of Moloney murine sarcoma virus (Mo-MuSV) was analysed by gel transfer hybridization. Genomic sizes of 4.6 kilobases (kb) for the ml-MuSV isolate, 5.2 kb for the m3- and 124-MuSV, 6.1 kb for the HTl-MuSV and 6.7 kb for the 78-Al-MuSV were determined. With the exception of the ml strain, subgenomic RNA species were found in cells infected by the various isolates. However, no common subgenomic RNA containing v-mos sequences could be characterized. Each transformed cell line expressed a different set of viral RNA species in terms of size and structure.

Characterization of Viral RNA in Cells Transformed by Various Isolates of Moloney Murine Sarcoma Virus