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With the aid of 11 neutralizing monoclonal antibodies which were investigated in four different neutralization tests, evidence was provided that several unrelated epitopes for neutralizing antibodies exist on the surface of poliovirus type 1. All monoclonal antibodies were able to neutralize poliovirus infectivity prior to and after virus adsorption to host cells. The quantitative enzyme-linked immunosorbent assay is introduced as a second independent system for the determination of antibody specificity. Antibodies could be divided into four groups according to their reaction patterns.

journal of general virology

Evidence for Several Unrelated Neutralization Epitopes of Poliovirus, Type 1, Strain Mahoney, Provided by Neutralization Tests and Quantitative Enzyme-linked Immunosorbent Assay (ELISA)