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SommaireLe present travail revele par des methodes cytochimiques les sites d'activite des deshydrogenases lactique, isocritrique, succinique, malique et glucose-6-phosphatique dans des fibroblastes d'embryons de poulets cultives in vitro et infectes par un virus oncogene, le virus du sarcome de Rous (souche schmidt-ruppin).Avec ce materiel, etudie pour la premiere fois par ces methodes, il a ete constate que ces sites sont toujours plus nombreux et plus intensement colores dans les fibroblastes ‘transformes’ que dans des fibroblastes normaux en division ou infectes seulement par un virus du type ‘Rous associated virus’ (RAV).Ces resultats laissent a penser que, dans le cas du sarcome de Rous de poulet et dans les conditions des experiences, la forte glycolyse des cellules malignes ne s'accompagne pas d'un affaiblissement des systemes enzymatiques respiratoires consideres.SummaryThe present work reports on the sites of lactic-, isocitric-, succinic-, malic- and glucose-6-phosphatic dehydrogenases in chick embryo fibroblast cultures infected with Rous sarcoma virus (schmidt-ruppin strain) using cytochemical methods.It was found that in RSV transformed cells reactive sites are both more numerous and appear more heavily stained than uninfected, dividing fibroblasts or fibroblasts infected with two other viruses of the avian leukosis group (RAV 1–2).These results seem to indicate that, in the case of Rous sarcoma virus infection of the chicken and under these experimental conditions, the glycolysis of the transformed (neoplastic) cells is not accompanied by a decrease of the respiratory enzymes studied here.

Mise en Evidence de Certaines Activites Deshydrogenasiques dans des Fibroblastes Aviaires Transformes in vitro par le Virus du Sarcome de Rous