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An assay for antiviral antibody based on antibody-dependent plaque enhancement (ADPE) in the macrophage cell line P388D1 is described which is as sensitive as, or more sensitive than a radioimmune assay. The method is applicable to a range of Togaviridae and Bunyaviridae although not to Picornaviridae and Herpesviridae. The variables affecting the assay are investigated.

Antibody-dependent Enhancement of Plaque Formation on Cell Lines of Macrophage Origin -- A Sensitive Assay for Antiviral Antibody