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SummaryCell cultures of the alfalfa looper, Trichoplusia ni (Tn-368), codling moth, Laspeyresia pomonella (Cp-169) and viper spleen cells (viper-VSW) were inoculated with Autographa californica non-occluded nuclear polyhedrosis virus (AcMNPV-NOV). In both Tn-368 and Cp-169 cells an increase in the concentration of virus protein was detected by ELISA from 9 to 20 h after virus inoculation. An average of 2 ng AcMNPV protein was detected in homogenates of T. ni larvae from 12 to 14 h post-infection (p.i.). HzSNPV proteins were first detected in Heliothis zea larvae 16 h p.i. At this time approx. 60 ng HzSNPV protein was detected in homogenates of the virus-infected larvae. Virus protein was not detected in viper spleen cells, and Lymantria dispar larvae inoculated with AcMNPV, or in Estigmene acrea larvae inoculated with HzSNPV.

Detection of Baculovirus Protein in Cell Culture and Insect Larvae by Enzyme-linked Immunosorbent Assay (ELISA)