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A mouse hybridoma cell line (Mo56) secreting IgG antibody to poliovirus type 3 D-antigen was obtained by fusion of a mouse myeloma cell line with spleen cells from mice immunized with Saukett virus. The monoclonal antibody was specific for Saukett virus strains in virus-neutralization and single-radial-diffusion tests. In immunoprecipitation tests the monoclonal antibody reacted with intact infectious virus particles (155S) and with a previously undescribed 70S poliovirus particle with D-antigenic reactivity.

A Hybridoma Cell Line Secreting Antibody to Poliovirus Type 3 D-Antigen: Detection in Virus Harvest of Two D-Antigen Populations