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The multiplication of rubella virus in tissue culture cells results in the production of two complement fixing antigens (Schmidt et al. 1968), two antigens detectable by immunodiffusion (Le Bouvier, 1969), an antigen detectable by haemagglutination and infective virions heterogeneous with respect to buoyant density (Magnusson & Skaaret, 1967). The physical characteristics of rubella virus haemagglutinin have been studied by equilibrium density gradient centrifugation in caesium chloride and values of 12.5 (Palmer & Murphy, 1968) and 1.33 (Amstey, Hobbins & Parkman, 1968) have been obtained. Strain differences in buoyant density have been noted for herpes simplex virus (Roizman & Roane, 1961). As part of a continuing study of rubella strains (Oxford, 1969), we have determined the buoyant density in caesium chloride of the haemagglutinin prepared from four virus strains with widely varying numbers of tissue culture passes.

A Difference in the Buoyant Density of Haemagglutinin From Rubella Virus Strains