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Murine mammary tumour virus (Bittner virus) is generally detected and titrated by in vivo tests. The time required for these tests varies from about 4 months to more than 1 year according to the clinical criteria accepted (Nandi, 1963). Recently Charney, Pullinger & Moore (1968) reduced this time to about 3 months by inoculation of newborn female mice and the detection of Bittner virus antigens in the milk of young adults by immunodiffusion tests. The immunological technique alone does not discriminate between infective and non-infective Bittner virus antigens. A faster in vitro assay would speed up the research of many aspects of the virus, including those at the cellular and molecular levels. Biological effects of Bittner virus preparations in vitro have been described, but an in vitro assay does not yet exist.

The Growth Accelerating Effect of Bittner Virus in Monolayers of Baby Mouse Kidney Cells