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SummaryThe Amicon TCIR recycle system with Diaflo PM30 filters provided a rapid, simple and inexpensive method for the concentration of baboon endogenous virus from a large quantity of culture medium from chronically infected cells. The recovery of infectivity and virion RNA-dependent DNA polymerase activity was substantially better than after the conventional concentration methods of ultra-centrifugation or precipitation with ammonium sulphate or polyethylene glycol 6000. The combination of ultrafiltration and Ficoll gradient centrifugation is recommended as a general procedure for the purification of fragile C-type viruses.

journal of general virology

A New, Rapid Procedure for the Concentration of C-type Viruses from Large Quantities of Culture Media: Ultrafiltration by Diaflo Membrane and Purification by Ficoll Gradient Centrifugation