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EBNA-containing chromatin from Raji cells was solubilized by treatment with high concentrations of urea and salt and fractionated by hydroxyapatite chromatography. Fractions eluting at different phosphate concentrations were analysed for the presence of EBNA by means of an anti-EBNA-specific 125I-labelled IgG absorption assay. The antigen was found in fractions containing non-histone chromatin proteins.

journal of general virology

Studies of Epstein-Barr Virus (EBV)-associated Nuclear Antigen: Solubilization from Raji Cell Chromatin with 5 M-Urea-2 M-NaCl and Fractionation on Hydroxyapatite