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When subjected to electrophoresis at 33 degrees C in 3% polyacrylamide gels with no urea added, the nucleoprotein and neuraminidase genes of an H2N2 and H3N2 virus migrate as RNA bands 5 and 6 respectively. If the temperature of electrophoresis is increased to 46 degrees C, however, this order of migration is reversed.

journal of general virology

Effect of Temperature on the Order of Electrophoretic Migration of Influenza Virus Neuraminidase and Nucleoprotein Genes in Acrylamide Gels Lacking Denaturing Agents