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SummaryRotavirus-infected intestinal epithelial cells in vivo and calf kidney cells in vitro have been examined by electron microscopy. Morphogenesis takes place in the cytoplasm and several particle types are observed. These can be classified broadly into two groups, one of which appears to represent the characteristic normal development of infectious virus and the other, distinctly different, which may be a non-infectious form. Two of the particle types are tentatively identified as the ‘single’ and ‘double’ capsid rotavirus particles seen typically in negatively stained preparations.

Different Particle Types in Tissue Culture and Intestinal Epithelium Infected with Rotavirus