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SummaryTreatment of purified preparations of tobacco rattle virus with 2 mm-EDTA decreased their infectivity, and that of RNA extracted from them, by about 90%. The effect of EDTA was apparently to remove divalent metal ions from the virus particles, making the RNA inside accessible to attack by a ribonuclease present in the preparations. Provided the enzyme activity was inhibited while the ions were absent, they could be put back with full restoration of full stability of infectivity.

Inactivation of Tobacco Rattle Virus by EDTA, and the Role of Divalent Metal Ions in the Stability of the Virus