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SUMMARY The polypeptides of BSCt cells infected with vaccinia virus and pulse labelled with (l~C)-protein hydrolysate or (35S)-methionine have been examined by discon- tinuous polyacrylamide gel electrophoresis followed by autoradiography. About 80 virus induced polypeptides were detected, 30 appearing before and 50 after the onset of virus DNA synthesis. These were termed pre- and post-replicative poly- peptides respectively. The synthesis of most pre-replicative polypeptides was turned off shortly after the peak of virus DNA synthesis; when virus DNA synthesis was inhibited this turn-off did not occur and post-replicative polypeptides were not made. The synthesis of some post-replicative polypeptides started at about the time of maximal DNA synthesis, reached a peak about I h later, and was then turned off. Other post-replicative polypeptides whose synthesis started at this time were made for prolonged periods. The synthesis of most post-replicative poly- peptides started about I h after the time of maximal virus DNA synthesis and continued thereafter for prolonged periods. The stability of pre- and post-replica- tive polypeptides was examined in pulse-chase experiments; most pre-replicative and 'early' post-replicative polypeptides were stable for prolonged periods, whereas, during a chase, eleven 'late' post-replicative polypeptides disappeared and seven new polypeptides appeared.

Vaccinia Virus Polypeptide Synthesis: sequential Appearance and Stability of Pre- and Post-replicative Polypeptides