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SummaryUsing indirect immunofluorescence and in situ hybridization techniques the synthesis of structural proteins (hexon and fibre), T-antigen and virus DNA were studied in infected rat embryo cells early after infection by adenovirus type 2. The data obtained from this study, including infectious virus yields, indicate that this is a semi-permissive system in which transformation occurs. The adenovirus type 2/rat embryo transformation system was found to be reproducible and the transformation frequency was influenced by the tissue of origin and not by the strain of rat. A comparison of the in vitro characteristics of cells transformed by either adenovirus type 2 (Ad-2) or type 12 (Ad-12) is reported. No obvious property was observed which would explain the different in vivo behaviour of rat cells transformed by these adenovirus serotypes.

journal of general virology

Interactions of Adenovirus Type 2 with Rat Embryo Cells. Permissiveness, Transformation and in vitro Characteristics of Adenovirus Transformed Rat Embryo Cells