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SUMMARY The kinetic data obtained from examination of restriction enzyme fragments give a direct determination of the position of the origin of replication in polyoma virus DNA. The results obtained are consistent with the previous estimates based on electron microscopic measurement of replicating molecules cleaved by endonuclease EcoRx (29 _+ 2 °/o from the EcoR1 site). The position of the ter- minus is diametrically opposite the origin, at approx. 79 % clockwise from the EcoR1 site.

Location of the Origin and Terminus of Replication in Polyoma Virus DNA