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SummaryMouse L cell interferon that had been either partially or completely inactivated by heating at 56 °C, boiling at 100 °C, shaking, repeated freezing and thawing, or treatment with mercaptoethanol and urea could be partly reactivated by addition of the anionic detergents, decyl-, dodecyl-, or tetradecyl sulphate. For complete reactivation it was necessary to disrupt disulphide bonds of interferon in solutions containing detergents.

Renaturation of Inactivated Interferons: requirement for Reduction of a Major Component; lack of Requirement for Reduction of a Minor Component